منابع مشابه
High-resolution cryo-EM proteasome structures in drug development
With the recent advances in biological structural electron microscopy (EM), protein structures can now be obtained by cryo-EM and single-particle analysis at resolutions that used to be achievable only by crystallographic or NMR methods. We have explored their application to study protein-ligand interactions using the human 20S proteasome, a well established target for cancer therapy that is al...
متن کاملCryo-EM structures of two bovine adenovirus type 3 intermediates.
Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are localized in the fivefold vertex region, while their facet structures are identical...
متن کاملCryo-EM enters a new era
Advances in detector hardware and image-processing software have led to a revolution in the use of electron cryo-microscopy to determine complex molecular structures at high resolution.
متن کاملCryo-EM structure of a transcribing cypovirus.
Double-stranded RNA viruses in the family Reoviridae are capable of transcribing and capping nascent mRNA within an icosahedral viral capsid that remains intact throughout repeated transcription cycles. However, how the highly coordinated mRNA transcription and capping process is facilitated by viral capsid proteins is still unknown. Cypovirus provides a good model system for studying the mRNA ...
متن کاملCryo-EM of viruses and vaccine design.
Knowledge of the atomic resolution structures of viruses can be a powerful tool for vaccine discovery and design. X-ray crystallography has long served as an invaluable method for virus structure determination at high resolution, but over the past decade, cryoelectron microscopy (cryo-EM) has begun to emerge as a complementary method that can also provide this kind of information. Crystallograp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Structural Biology
سال: 2018
ISSN: 1047-8477
DOI: 10.1016/j.jsb.2018.10.004